Composite
shTERTtRNA

Part:BBa_K1722011:Design

Designed by: Zhiwei Zhang   Group: iGEM15_SZU_China   (2015-09-02)

shTERT+tRNA(CUA) Composite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Both shTERT promoter and tRNA are achieved from Shenzhen Second People's Hospital. We designed primers and amplified the gene sequences from psi-Check2 vector. Using 3A Assembly method, we constructed hTERT and tRNA in pSB1C3.

Source

Both shTERT promoter and tRNA are achieved from Shenzhen Second People's Hospital.

References