Composite
shTERTtRNA
Part:BBa_K1722011:Design
Designed by: Zhiwei Zhang Group: iGEM15_SZU_China (2015-09-02)
shTERT+tRNA(CUA) Composite
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Both shTERT promoter and tRNA are achieved from Shenzhen Second People's Hospital. We designed primers and amplified the gene sequences from psi-Check2 vector. Using 3A Assembly method, we constructed hTERT and tRNA in pSB1C3.
Source
Both shTERT promoter and tRNA are achieved from Shenzhen Second People's Hospital.